Fly TFBS Cluster Analysis

STEP 1: Enter target (co-expressed) genes

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Paste gene IDs (max. 5000 gene IDs):




OR upload a file containing a list of gene identifiers (max. 5000 gene IDs):

STEP 2: Enter background genes

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All 14832 genes in the oPOSSUM database

OR a random set of  genes from the oPOSSUM database

OR paste gene IDs:



OR upload a file containing a list of gene identifiers:

STEP 3: Select transcription factor binding site cluster parameters

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TFBS Cluster Selection Based on TF Families

All TFBS clusters
OR select specific TFBS cluster families:
AT-hook Arid BetaBetaAlpha-zinc finger Brinker CP2 DM E2F E2F T Ets Forkhead GATA Glial Cells Missing (GCM) Helix-Loop-Helix High Mobility Group Homeo Homeo::Nuclear Factor I-CCAAT-binding Hormone-nuclear Receptor IRF LAG1 Leucine Zipper Loop-Sheet-Helix MADS MH1 Myb NFY CCAAT-binding Other RFX Rel Runt Sand Stat T TATA-binding Unknown Zeste

STEP 4: Select TFBS cluster search parameters

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Conservation cutoff:
0.40 0.60

Matrix score threshold:
75% 80% 85% OR enter score threshold % (min. = 75%)

Amount of upstream / downstream sequence:
3000 / 3000 3000 / 2000 2000 / 2000 2000 / 1000 1000 / 1000 1000 / 0 OR enter custom values / bp (max. = 3000 / 3000)

Number of results to return:
Top 5 10 20 All results
OR only results with Z-score >= 5 10 15 and Fisher score >= 5 7 9 (Default values have been chosen based on empirical studies)

Sort results by:
Z-score Fisher score

Please enter your email address:

Press the Submit button to perform the analysis or Reset to reset the analysis parameters to their default values.
Depending on parameters selected and server load, the analysis may take anywhere from a minute to a few hours to complete.